robosep human cd14 positive kit Search Results


cd14 microbeads  (Miltenyi Biotec)
98
Miltenyi Biotec cd14 microbeads
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Cd14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
cd14 microbeads - by Bioz Stars, 2026-05
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robosep human cd14 positive kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep human cd14 positive kit
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep Human Cd14 Positive Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
robosep human cd14 positive kit - by Bioz Stars, 2026-05
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robosep  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/robosep/product/STEMCELL Technologies Inc
Average 90 stars, based on 1 article reviews
robosep - by Bioz Stars, 2026-05
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automated cell isolator (robosep  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc automated cell isolator (robosep
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Automated Cell Isolator (Robosep, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automated cell isolator (robosep - by Bioz Stars, 2026-05
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cd14 selection kits  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd14 selection kits
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Cd14 Selection Kits, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd14 selection kits - by Bioz Stars, 2026-05
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robosep buffer  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep buffer
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep Buffer, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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robosep negative human monocyte kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep negative human monocyte kit
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep Negative Human Monocyte Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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robosep device  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep device
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep Device, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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robosep device - by Bioz Stars, 2026-05
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robosep instrument  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc robosep instrument
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Robosep Instrument, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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robosep instrument - by Bioz Stars, 2026-05
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cd3+ negative selection kit  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd3+ negative selection kit
Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, <t>CD14+</t> cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).
Cd3+ Negative Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, CD14+ cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).

Journal: Biology

Article Title: Mitochondrial Ultrastructure and Activity Are Differentially Regulated by Glycolysis-, Krebs Cycle-, and Microbiota-Derived Metabolites in Monocytes

doi: 10.3390/biology11081132

Figure Lengend Snippet: Lactate, succinate, fumarate, butyrate, and acetate induce mitochondria polarization in monocytes. Mitochondrial membrane potential (Δψ m ) was assessed in human monocytes by flow cytometry, using the tetramethylrhodamine methyl ester molecular probe. For analysis, CD14+ cells were gated and TMRM mean fluorescence intensity (indicative of Δψ m ) was recorded. ( A ) shows the Δψ m fold change at 1, 2, 3, and 4 h post metabolite exposure, ( B ) shows representative histograms of TMRM fluorescence. Results are from eight independent experiments. Mitochondrial polarization was statistically significant ( p < 0.001, Kruskal–Wallis and Dunn’s post hoc tests).

Article Snippet: PBMC were subjected to several low-speed washes in PBS in order to minimise platelet contamination and were subsequently subjected to monocyte enrichment by negative selection using a monocyte-enrichment cocktail and magnetic separation on an EasySep magnet or RoboSep instrument (Stem Cell Technologies, Vancouver, BC, Canada) or by positive selection using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Membrane, Flow Cytometry, Fluorescence